Anti-human IgE mAbs (107/182/101), biotin

Anti‑human IgE mAbs (107/182/101), biotin

3810-8-250

Publications: 6
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Validated for our assays.

Content

Monoclonal antibodies 107/182/101, biotinylated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

In stock

Delivery 4-9 business days

Shipping $0

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Product specifications

Intended use

These monoclonal enable specific detection of human IgE in ELISpot.

Serum/Plasma samples

Recommendation

107/182/101 are recommended as detection mAbs in ELISpot in combination with capture mAb 107 (product code 3810-3).

Product details

ProductAnti-human IgE mAbs (107/182/101), biotin
ApplicationELISpot
AnalyteIgE
Antibody107/182/101
ConjugateBiotin
ClonalityMonoclonal
ImmunogenNative human IgE
HostMouse
IsotypeIgG1/IgG2b/IgG2a
ReactivityHuman
Specificity

Human IgE

Purification

Purified from in vitro cultures by protein G affinity chromatography.

Biotinylation

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Concentration1 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Monoclonal antibodies 107/182/101, biotinylated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.

Selecting the appropriate mAbs is key to detecting both total and antigen-specific human IgA. This blog post explores Mabtech’s mAbs, tailored for ELISpot to detect human IgA antibody-secreting cells.

 

Application guide: ELISA

 

Antigen-specific IgE

Coating:
Antigen
Detection mAbs:
107/182/101, biotin

IgE Ag

Total IgE

Coating:
107, unconjugated
Detection mAbs:
182, biotin

IgE total

 

Application guide: ELISpot

 

Total IgE

Coating mAb:
107, unconjugated
Detection mAbs:
107/182/101, biotin

ELISpot IgE

 

 

Get inspired!

Make sure to check out how researchers have used Mabtech's human IgE ELISA, ELISpot, and FluoroSpot kits in allergy research. You can find it all in our Publication database.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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IgE

Analyte description

The main physiological function of immunoglobulin E (IgE) is to protect against parasitic infestation. However, IgE is also associated with allergy, and allergen-specific IgE antibodies may trigger allergic reactions in the presence of the allergen. IgE is normally found in low amounts in serum/plasma but is significantly increased in allergic individuals, and the detection of allergen-specific IgE serves as the basis for many in vitro assays for allergy diagnosis.

Alternative namesImmunoglobulin E, IgE
Cell typeB cell
Gene ID3497
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